For ALL research questions regarding Core usage, contact:
Robert A. Edwards, M.D., Ph.D.
Director, Pathology Research Services Core
Med Sci D435
email: redwards@uci.edu
phone: 949-824-8576
1. Histology and Immunohistochemical Staining
SERVICES OFFERED
Services provided by this component of the Core include routine histology (tissue trimming, embedding, sectioning, including frozen sections, H&E staining, histochemical staining and immunohistochemical staining), and the retrieval of paraffin-embedded blocks of normal and diseased tissue (by diagnosis) from our archives. A detailed list is provided on the order form.
COMPLIANCE
IRB or IACUC approval is required for all services requiring the use of human or animal tissue, respectively. Service requests will NOT be processed without the necessary compliance information (provide copy of appropriate approval form to Dr. Carpenter; also see COMPLIANCE guidelines).
COST TO INVESTIGATOR
Re-charge rates are listed on the order form.
ORDERING SERVICES
Select either the PDF or WORD version of the Histology service order form, complete the form and include a paper copy with your samples (described below). Fax (714-456-5873) or email to Jeffrey Kim in Histology (jbkim@uci.edu).
Investigators may use our departmental courier service to send samples to UCIMC, where the Histology unit is located. Processed samples can also be returned by courier. To send samples via courier, email the request form to Jeffrey Kim (specifying that samples will follow via courier) and include a copy of the request form with the samples. Pack the samples securely in a sturdy box. Tightly seal any containers filled with formalin. Address the box to Pathology Research Services Core, c/o of Jeffrey Kim, Bldg. 1, UCIMC.
Be sure to specify that samples are to be returned by courier also. Take the box to the courier pickup location in the Med Sci D426 (inside Dr. Edwards' lab) between 9 A.M. and 6 P.M. on the School of Medicine campus. Jeffrey Kim will send you an email on the day that samples are sent back to Med Sci D426 drop by return courier.
Contact Dr. Edwards with questions about the service.
2. Morphometry and Image Analysis of Tissue Sections
ASSOC. DIRECTOR
Phil Carpenter, Professor of Clinical Pathology
email: pmcarpen@uci.edu
phone: 714-456-6141
fax: 714-456-5873
SERVICES OFFERED
This component of the Core provides services required to quantify cellular elements or molecular signals detectable by microscopic evaluation of tissue sections. This includes determining the number and/or size of cellular organelles; measuring the intensity of immunostaining of individual cells in a tissue section, and counting the percentage of cells and/or nuclei that are immunostained with a particular antibody. These analyses can be performed on sections which were H&E stained or immunostained in the Histology component of the Core, or on sections prepared by individual investigators.
COMPLIANCE
IRB or IACUC approval is required for all services requiring the use of human or animal tissue, respectively. Service requests will NOT be processed without the necessary compliance information (provide copy of appropriate approval form to Dr. Carpenter; also see COMPLIANCE guidelines).
COST TO INVESTIGATOR
The re-charge rate for this service is $18 per hr of data acquisition.
ORDERING SERVICES
Contact Dr. Carpenter directly to arrange services.
3. Laser-Capture Micro-Dissection of Tissue Sections
ASSOC. DIRECTOR
Robert Edwards, Associate Professor of Pathology
email: redwards@uci.edu
phone: 949-824-8576
FAX: 949-824-2160
SERVICES OFFERED
This component of the Core allows investigators to fractionate
cell populations from tissue sections. For example, normal tissue can
be purified from tumor tissue on tissue sections containing a mixture
of these cell types. The core facility, located in Medical Sciences I, D426C, contains
a Leica CM3050 cryostat and a Leica LS-AMD outfitted with fluorescent
optics (allowing the selective visualization and capture of immunostained
µm. For investigators whose work requires an Arcturus XT, with UV and IR laser capture modes, to facilitate the capture of single cells (and live cells) with minimal damage to biomacromolecules may be used instead. This instrument is located in the CLRB (Building 54, 4th floor) at the UCIMC campus in Orange.
This service can be performed on sections that were H&E stained in the Histology component of the Core, or on sections prepared by individual investigators.
For vendor info on instruments or Supplies:
Leica
Links to LCM Protocols:
Leica Sample Prep
COMPLIANCE
IRB or IACUC approval is required for all services requiring the use of human or animal tissue, respectively. Service requests will NOT be processed without the necessary compliance information (see order form and COMPLIANCE guidelines; provide copy of appropriate approval form to Dr. Edwards).
COST TO INVESTIGATOR
All users must first undergo a two hour orientation on the instrument with Dr. Edwards. There is no fee for UCI investigators, and $100 per hour for non-UCI affiliated users. All investigators requiring the services of a surgical pathologist to perform the microdissection will be charged $150 per hour of LCM time. Investigators must purchase appropriate caps with laser-capture membrane (Arcturus), or foiled-coated slides (Leica).
ORDERING SERVICES
Contact Dr. Edwards to arrange services. Users performing their own microdissections must receive training on the use of the LCM from Dr. Edwards. LCM time must be reserved in advance and each session must be recorded in the log book.
4. Tissue Bank and Procurement Service (TBPS)
ASSOC. DIRECTOR
Dan Mercola, Professor of Pathology
email: dmercola@uci.edu
phone: 949-824-1298
FAX: 949-824-2160
SERVICES OFFERED
The Core will bank remnants of tissue (both normal and tumor) resected from patients undergoing surgery at UCIMC. Remnant tissue is the residual portion of a surgical specimen following processing for diagnostic purposes (see Tissue Custody Policy). Signed consent from each patient will also allow us to obtain peripheral blood samples (serum and DNA) and clinical follow-up at 6 month intervals for 3 years. Investigators may request access to tissue and blood samples, as well as clinical data. Requests for large amounts of tissue or multiple samples, or access to relatively rare specimens will be reviewed by the Core’s Tissue Use Committee.
COMPLIANCE
IRB approval is required for access to this tissue. Service requests will NOT be processed without the necessary compliance information (see order form and COMPLIANCE guidelines; provide copy of appropriate approval form to Dr. Mercola). Investigators are encouraged to contact Dr. Mercola concerning the availability of tissue prior to submitting projects for IRB approval.
COST TO INVESTIGATOR
Re-charge rates are listed on the order form.
ORDERING SERVICES
For previously approved requests, select either the PDF or WORD version of the Tissue Bank request form, complete the form and fax (714-456-5873) or email to Anne Sawyers (asawyers@msx.ndc.mc.uci.edu), Clinical Coordinator for the Tissue Bank. Contact Ms. Sawyers or Dr. Mercola with questions about the service.
5. Genotyping (includes Nucleic Acid Extraction From Blood and Tissue Sections)
ASSOC. DIRECTOR
Robert Edwards, Associate Professor of Pathology
email: redwards@uci.edu
phone: 949-824-8576
FAX: 949-824-2160
SERVICES OFFERED
Genotyping services are performed in the Diagnostic Molecular Pathology lab and include a variety of PCR and/or Southern blot based analyses of mutant or polymorphic alleles. The lab will work with investigators to identify genotype-based tests that may be of value in clinical treatment and cancer control studies. This service can also perform mRNA-based testing for occult cancer or residual disease. In addition, this component of the Core provides automated nucleic acid extraction from whole blood, paraffin-embedded or frozen tissue sections.
COMPLIANCE
IRB or IACUC approval is required for all services requiring the use of human or animal tissue, respectively. Service requests will NOT be processed without the necessary compliance information (provide copy of appropriate approval form to Dr. Edwards).
COST TO INVESTIGATOR
Nucleic acid extraction re-charge rates are listed on the order form. Re-charge rates are determined on a case-by-case basis in consultation with Dr. Edwards or the UCI Clinical Research Finance Administration (for specimens processed as part of a UCI clinical research study).
ORDERING SERVICES
Contact Dr. Edwards to arrange specific genotyping analyses. For nucleic acid extraction services, select either the PDF or WORD version of the Nucleic acid service order form, complete the form and fax (714-456-4936). Contact Dr. Edwards with questions about the service.