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New Test for BCR-ABL1 MINOR (p190), Quantitative
The UC Irvine Department of Pathology and Laboratory Medicine will replace the current test for monitoring BCR-ABL Minor (p190) transcript levels with a new lab-developed quantitative assay.

Our current vendor, Asuragen, is discontinuing their multiplexed BCR-ABL Quant reagents and calibrators. The new test kit has better sensitivity (0.0025% ratio).

Methodology and reporting:
The BCR-ABL p190 (Minor) quantitative assay is a Reverse Transcription-Real Time qPCR method designed to measure e1a2 fusion transcript (p190 fusion protein) in total RNA. Expression levels for p190 fusion transcript are expressed as a percentage of BCR-ABL1 expression to the expression of the control gene ABL1. This assay will not detect the e13a2 and e14a2 fusion transcripts that encode the p210 fusion protein.

The new test (Test code: BCR190) will replace the current test for BCR-ABL P190 (Test code: BCRMIN). For p210 quantitative testing, please order BCR-ABL1 Major Breakpoint Quantitative Test (Test code: BCR210). To determine the type of BCR-ABL1 translocation (p210 vs. p190), please order BCR-ABL Qualitative Test (Test code: BAQUAL).

Effective date: Sept. 19, 2018

Specimen requirement and testing information:

Specimen: Whole blood or bone marrow

Test methodology: Reverse Transcription-Real Time qPCR

Test code: BCR190

Ambient: up to 2 hours from collection time
Refrigerated: 24 - 72 hours (must be received by Molecular Pathology lab within 72 hours of collection)
Frozen: Unacceptable

Turnaround time (TAT):
STAT: Not available
Routine: 5 - 7 days
Set-up days: Once a week

Rejection criteria:
Specimens that exceed stated stability, gross hemolysis, unlabeled/mislabeled/mismatched specimens, leaking collection tubes and incomplete requisition forms

A summary of all tests offered by our laboratory services can be found here:


Jeff Chan, MD, PhD
Director, Diagnostic Molecular Pathology Laboratory

Edwin S. Monuki, MD, PhD
Chair, Department of Pathology & Laboratory Medicine
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